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Size: 1024KBCorrelation between the electron-dense amorphous coating of the synaptic vesicles and the release of the synaptic vesicle contents, mediated by Ca2+ and other factors, in frog nerve-muscle synapses. The release of neurotransmitter (NT) is dependent on Ca2+ (Ca2+-transmission) and is accompanied by a close apposition of the synaptic vesicles (SV) and the plasmalemma (P-face). This close apposition, defined as the electron-dense amorphous coating, has been considered to be a morphological feature of SV that leads to the release of NT. To test this hypothesis, we performed a double-label immunoelectron microscopy study using pre- and postsynaptic antigens, including SV-associated SV2 (antibody produced by Merritt [1985] Proc. Natl. Acad. Sci. U.S What's New in the? 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